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. 2018 Jun 27;27(7):1047–1067. doi: 10.1177/0963689718779346

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Endogenous gene expression markers in established cell clones of the iPSC 194 cell line: (a) OCT4, (b) SOX2, (c) NANOG, (d) c-MYC (typical of pluripotent cells), (e) MyoD (skeletal myoblast marker), and (f) BRACHYURY (mesoderm marker). Human embryonal cells (ESC P27) served as a positive control. The expression of the studied genes was normalized to the expression of two housekeeping genes (ACTB and GAPDH). Samples: SkMC 194/6: skeletal myoblast cells from patient no. 194 after the sixth passage; iPSC 194 cl. 10/11/13: clones 10, 11, and 13 of the induced pluripotent stem cell line no. 194 of myoblastoid origin; ESC P27: embryonal stem cell line after the 27th passage. Values are given as means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001. ACTB: β-actin; c-MYC: cellular c-Myc oncogene product; ESCs: embryonic stem cells; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; iPSCs: induced pluripotent stem cells; MyoD: myogenic differentiation 1; OCT4: octamer-binding transcription factor 4; SOX2: sex determining region Y - box 2.