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. 2018 Jun 27;27(7):1047–1067. doi: 10.1177/0963689718779346

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 12. — Cardiac gene expression in generated SMiPSC-CMs on days 20 and 40 of in vitro culture: (a) TNNT2, (b) NKX2.5, (c) TNNI1, (d) TNNI3, (e) α-MHC, (f) β-MHC, (g) KCNJ2, (h) CX43, and (i) SERCA 2a. ACTB and GAPDH gene expression was used to normalize the examined gene expression levels. Samples: SMiPSC 194: 194 line of induced pluripotent stem cells of skeletal myoblast origin as a negative control; SMiPSC-CMs 20/40 day: differentiated SMiPSC-derived cardiomyocytes on days 20 and 40 of in vitro cell differentiation culture; adult heart: sample collected from ventricular heart muscle as a positive reference. Plot: mean value + SD.