Figure 15.
Comparison of diverse transient kinetics of Ca2+ in 20-day SMiPSC-CMs under isoproterenol treatment. (a) Demonstration of a faulty calcium turnover pattern in 20-day iPSC-derived cardiomyocytes after isoproterenol stimulation in two analyzed cell culture areas. Clusters with slowed contractions (36 beats per minute on average) and disturbed calcium flow, including its uptake from the cytoplasm, a long plateau phase (lasting up to 1 second), and a slower calcium decay time reflect impairment of the contractile machinery. (b) The normal course of calcium transient morphology for another fragment of the examined cell culture surface. Measurements were conducted within 1 minute of fluorescent signal registration.