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Immunostaining on days 20 and 40 of cardiac differentiation in in vitro culture of NKX2.5 (an early cardiac differentiation marker), TNNT2 (cardiac troponin) ((a) and (b)) and α-MHC (myosin heavy chain α) (cardiac-specific marker), CNX43 (intercellular junction marker) ((c) and (d)). Scale bar is 50 μm.
