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. 2018 May 21;46(12):6271–6284. doi: 10.1093/nar/gky413

Figure 4.

Figure 4.

HPLC separation and quantification of the ribonucleosides obtained after enzymatic hydrolysis of synthesized RNAs. (A) Chromatogram of the standard solutions of the four ribonucleosides (adenosine, guanosine, uridine and cytidine at concentrations of 0.25 mM each in the digestion buffer, the retention times and the base corresponding to each peak are displayed on top of each peak. (B) RNA hydrolysate obtained from RNA synthesis by CS13 and an equimolar mix of the four NTPs (C) RNA hydrolysate obtained from RNA synthesis by CS13 and a mix containing ATP/CTP/GTP/UTP at a molar ratio of 1:1:1:10. (D) RNA hydrolysate obtained from RNA synthesis by CS13 and an equimolar mix of ATP and CTP. (E) RNA hydrolysate obtained from RNA synthesis by CS13 and an equimolar mix of GTP and UTP. UV detection at 260 nm was used.