Figure 5.
Over-initiation in oriCallADP cells leads to increased sensitivity to replicative stress. (A and B) DNA histograms of wild-type oriC (A) or oriCallADP (B) cells growing in LB (top panels), or minimal media supplemented with glucose, casamino acids and uracil (bottom panels), treated with cephalexin and rifampicin, and analyzed by flow cytometry. The number of origins in the cells at the time of drug treatment is shown. (C) Survival fraction of cells incubated in the presence of AZT or HU are plotted after 18 h of growth. Two separate experiments, with triplicate plates were run. Error bars are SD of mean.