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siRNA versus plasmid penetration into organoids that are grown in Matrigel microbeads. Fluorescent siRNA penetration (A) or GFP plasmid expression (B) inside encapsulated RWPE-1 organoids was assessed using flow cytometry and spinning disk confocal microscopy (40× magnification, scale bar 100 μm) 3 days after electroporation with 20 nM fluorescent siRNA or GFP plasmid (*P < 0.05; **P < 0.01). False color imaging is used here for better visualization.
