Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 16;46(17):8993–9010. doi: 10.1093/nar/gky733

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 1. — Trypanosoma brucei 4EIP interacts with EIF4E1. (A) Conserved regions in kinetoplastid 4EIPs. Amino acids highlighted in black are identical in all aligned sequences. Residues highlighted in gray are partially conserved—either identical or chemically similar in a majority of sequences. Sequences are (in vertical order): Trypanosoma grayi DQ04_01981000; Trypanosoma theileri ORC92063.1; Trypanosoma cruzi TcCLB.508461.290; Trypanosoma congolense TcIL3000_9_4530 Tryanosoma brucei Tb927.9.11050; Trypanosoma vivax TvY486_0905070; Endotrypanum_monterogeii EMOLV88_350043200; Leishmania major LmjF.35.3980; Blechomonas_ayalai Baya_047_0070; Bodo caudatus CUG36708.1. (B) The photos show growth of yeast on plates with stringent selection for the interaction between bait and prey plasmids. The identities of the plasmids used are shown next to the streaked yeast. Full results with controls are shown in Supplementary Figure S1A. (C) Extracts were made from bloodstream-form trypanosomes with or without a V5- in situ tagged eIF4E1 and myc-tagged 4EIP. Anti-myc immunoprecipitates were subjected to sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. In: input extract from 5 × 10⁶ cells; U: unbound fraction from 5 × 10⁶ cells; E: eluate from immunoprecipitating beads, from 1 × 10⁸ cells. Upper panel: anti-myc pull-down, with cells lacking a myc-tagged protein as control. Lower panel: anti-V5 pull-down, with cells lacking V5-tagged protein as the control. (D) As in (C) but with anti-V5 as the precipitating antibody. (E) As in (C) but with procyclic-form trypanosomes. (F) Western blot showing relative amounts of V5-tagged 4EIP and EIF4E1 in bloodstream forms. The only band from 4EIP migrated at ∼37 kDa. The control is ribosomal protein S9.