Figure 4.

4EIP tethering suppresses translation and induces mRNA destruction in procyclic forms. (A) Effect of LambdaN-4EIP-myc expression on proliferation. A cumulative growth curve is shown. Results are arithmetic mean with error bars showing standard deviation from three assays. (B) Expression of LambdaN-…-myc fusion proteins. (B) Effects of different tethered reporters on CAT activity (left bars, green in online version) and mRNA (right bars, mauve in online version). Results are arithmetic mean with error bars showing standard deviation. (C) Time course of LambdaN-4EIP-myc expression in cells expressing CAT mRNA with or without boxB. A northern blot showing the effect on CAT mRNA is shown below, and quantitation of replicates is to the right. (D) Polysomes were separated on sucrose gradients, either without tetracycline (blue) or 6 h after tetracycline addition (red). Typical gradient profiles and northern blots are shown at the top, and quantitation of three replicates beneath with “+tet” on the right.