Figure 5.

RNAs bound to 4EIP are relatively unstable mRNAs and were classified as ‘bound’ if the reads per million in the fraction bound to TAP-4EIP were at least twice those in the flow-though in both biological replicates, and the minimum count number was 10. ‘Unbound’ mRNAs has a ration of <1 in both replicates. The mRNAs encoding ribosomal proteins (RP) were mostly not bound but were considered separately. All results are for bloodstream forms. (A) Ribosome density for all ‘bound’ and ‘unbound’ mRNAs, as judged by ribosome profiling (49). Medians are indicated, as are the Student’s t-test values. (B) mRNA half-lives for all ‘bound’ and ‘unbound’ mRNAs (47). (C) Open reading frame or coding sequence (CDS) lengths of all ‘bound’ and ‘unbound’ mRNAs. (D) As (A), but for a subset of length-matched mRNAs (Supplementary Table S3, sheet 4). (E) As (B), but for the subset of length-matched mRNAs. (F) As (C), but for the subset of length-matched mRNAs.