Table 2.
Technology | Number of units sequenced | Number of reads | N50 read length | Total amount of data in base pairs |
---|---|---|---|---|
Illumina, MiSeq | Part of one flow cell (300 bp paired-end) | 4 471 800 (read pairs) | 242 bp | 753 Mb |
PacBio, RS IIa | Four SMRT cells (P6-C4 chemistry) | 239 198 | 16.9 kb | 2.7 Gb |
ONT, MinIONb | Two flow cells (R9.5 cells, 1D2 library) | 34 167 | 44.9 kb | 1.6 Gb |
aStatistics based on extracted subreads. After length (>500 bp) and quality (0.83) filtering.
bStatistics after filtering for reads longer than 30 kb.