Figure 1.

Structures of DNMT3 proteins and MeCP2 and interaction of MeCP2 with DNMT3A. (A) Domain architecture of mouse DNMT3A, DNMT3L and MeCP2 annotated with the domain boundaries used in this work. (B) Western blot detection of His-DNMT3A after its pull-down with the GST-tagged N-terminal truncated MeCP2 (MeCP2ΔN, residues 104–486). (C) Western blot detection of Myc-tagged DNMT3A after its pull-down by EYFP-tagged MeCP2 following transient co-expression of both proteins in human HEK293 cells. As a negative control, the pull-down was performed after co-expression of DNMT3A with EYFP. See also Supplementary Figure S10. (D) Western blot detection of endogenous MeCP2 after its pull-down by DNMT3A using mouse brain protein extracts. An immunoprecipitation using an IgG isotype control was included as negative control. NTD, N-terminal domain.