Figure 1.
BMDMs from macrophage-specific DHX9 knockout mice display impaired innate immunity after DNA virus infection. (A) Loss of DHX9 protein in BMDMs, but not in BMDCs from LysM-cre Dhx9f/f mice was confirmed by western blotting. (B–I) BMDMs from Dhx9f/f and LysM-cre Dhx9f/f mice were mock-infected or infected with MHV-68 or HSV-1 at MOI 5 for 9 and 18 h. Total cellular RNA was extracted and subjected to RT-qPCR analysis of Il-6 (B, F), Ifn-β (C, G), Tnf (D, H), and Il-1β (E, I) mRNA levels. The data represent the mean ± SD of triplicate assays. *P < 0.05; **P < 0.01; ***P < 0.001 versus control, ns = nonsignificant (Student's t test).