Figure 5.

Nuclear DHX9 is not translocated to the cytosol after DNA virus infection in BMDMs and MEFs. (A) Two representative confocal-microscopy images of BMDMs that were either mock-infected (top) or infected with EGFP/MHV-68 (middle) or HSV-GFP (bottom) at MOI 5 for 12 h. (B) Two representative confocal-microscopy images of MEFs that were either mock-infected (top) or infected with EGFP/MHV-68 (middle) or HSV-GFP (bottom) at MOI 2 for 12 h. The cells were fixed and stained for endogenous DHX9 (Cy3-red), while DAPI staining (blue) was performed to visualize nuclei. Images were obtained by means of an LSM 700 confocal microscope from Carl Zeiss. All panels show × 1,000 magnification (scale bar: 20 μm).