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. 2018 Jul 28;46(17):8917–8925. doi: 10.1093/nar/gky673

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — EcoRI E111G–DNA complexes inhibit unwinding of DNA forks by RepΔ2B to a greater extent than by wild type Rep. (A) Native polyacrylamide gel of the products of unwinding a synthetic forked DNA structure bearing two EcoRI sites within the duplex region without and with addition of EcoRI. Wild type and mutant Rep enzymes were present at final concentrations of 1, 10, 50 and 100 nM and extent of unwinding analysed after a 10 min incubation. The position of the ³²P label on the DNA substrate is indicated with a star. (B) The fraction of forked DNA substrate unwound in the absence and presence of EcoRI for (i) wild type Rep and (ii) RepΔ2B. (C) The degree of inhibition of forked DNA unwinding effected by the addition of EcoRI.