Figure 1.

Effects of CIH on MnSOD and CuZnSOD levels in rat CB. MnSOD (A–C) and CuZnSOD (D–F) immunoreactivity (MnSOD-ir and CuZnSOD-ir, respectively) in CBs from rats exposed to sham conditions (A and D) and CIH (B and E) for 7 days. Inset, negative control from the same rat but without the primary antibody. Scale bar, 20 µm. Quantification of MnSOD positive staining (C) showed that CIH produced a significant increase of MnSOD-ir, **p<0.01 Student’s t-test, n=6 sham and n=4 CIH-rats. Quantification of CuZnSOD-ir (F) did not show statistical differences. p>0.05, Student’s t-test, n=4 rats per group. Abbreviations: CIH, chronic intermittent hypoxia; CB, carotid body.