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. 2018 May 18;66(10):753–765. doi: 10.1369/0022155418776229

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Figure 5. — Effects of CIH on 3-NT levels in rat carotid body and adrenal medulla. Photographs show the positive immunoreactivity for 3-NT (3-NT-ir) in the CB (A and B) and in chromaffin cells from adrenal the medulla (D and E), which correspond to high magnification images selected from adrenal medullas showed in left side of Fig. 2C. A and D, sham rats. B and E, CIH exposed rats. Inset, negative sham realized omitting the primary antibody. Scale bar, 20 µm. The exposure to CIH produced a significant increase in 3-NT-ir levels in the CB (C) and adrenal medulla (F). ***p<0.001, *p<0.05 compared with sham rats. Student’s t-test, sham n=10 and CIH n=4 for CBs; sham n=6, CIH n=7 for adrenal medulla. Abbreviations: CIH, chronic intermittent hypoxia; 3-NT, 3-nitrotyrosine; 3-NT-ir, 3-NT immunoreactivity; CB, carotid body.