Fig 2.

B355252 confers protection against CoCl₂-induced decreases in cell viability during hypoxia and reoxygenation A) Cell viability assay showing addition of B355252 protects against a CoCl₂-induced decrease in cell viability. HT22 cells were pretreated with the indicated concentrations of B355252 for 2h prior to adding 300 μM CoCl₂ for 24h. B) Cell viability assay showing addition of B355252 also mitigates damage that further occurs after CoCl₂ is removed. For this reoxygenation phase, cells were treated as in A, then washed and incubated with fresh, drug-free media for an additional 24h before assessing cell viability. One-way ANOVA followed by Bonferroni's post-test were used for analysis. ### p<0.001 vs. control. *** p<0.001. ** p<0.01 vs. CoCl₂ alone.