Fig 4.

B355252 protects against CoCl₂-induced alterations in mitochondrial fusion proteins OPA1 and Mfn2 A) Western blot of mitochondrial fusion protein, OPA1, and loading control, β-actin, in HT22 cells. Total protein lysates were obtained from untreated control and cells treated for 10 and 24hr with 300 μM CoCl₂ with or without 2.5 μM B355252. Picture is representative of three separate experiments. B) Quantification of average OPA1 expression when normalized to β-actin. C) Western blot of mitochondrial fusion protein, Mfn2, and loading control, β-actin, in HT22 cells. Protein samples were obtained as indicated in A and picture is representative of three separate experiments. D) Quantification of average Mfn2 expression when normalized to β-actin. One-way ANOVA followed by Bonferroni's post-test was used for analysis. ### p<0.001 vs. control. *** p<0.001 vs. CoCl₂ alone.