Fig 6.

B355252 reduces CoCl₂-induced autophagy A) Western blot of autophagy associated protein, Beclin-1, and loading control, β-actin, in HT22 cells. Protein samples were obtained as indicated in Figure 4A. Picture is representative of three independent experiments. B) Quantification of average Beclin-1 expression when normalized to β-actin. C) Western blot of autophagy associated protein, LC3-I/II in HT22 cells. Protein samples were obtained as indicated in Figure 4A and picture is representative of three separate experiments. D) Quantification of average LC3-II/LC3I ratio. Ratio was obtained by dividing the LC3-II fluorescent signal by the LC3-I fluorescent signal. One-way ANOVA followed by Bonferroni's post-test was used for analysis. ### p<0.001 vs. control. * p<0.05 vs. CoCl₂ alone. ** p<0.01 vs. CoCl₂ alone.