Fig 2.

AGK2 inhibited HBV replication in HepAD38 cell line. (A-B) HepAD38 cells were cultured with AGK2 (10μM) for seven days, and Entecavir (25nM) was used as positive control. The cells total RNA was extracted and the HBV 3.5kb RNA, total RNAs were analyzed via real-time PCR. The mRNA level of β-actin was used as an internal standard for quantification. *p <0.05. (C) Northern blot was applied to determine the HBV mRNA level. The rRNA level of 28s/18s was used as an internal standard. (D-E) HBV DNA replicative intermediates were extracted and subjected to absolute quantification PCR and Southern blot analysis, *p<0.05. (F) Western blot analyzed the HBc expression after seven days treatment, GAPDH was used as an internal standard. (G-H) HBsAg and HBeAg ELISA was used to screen culture supernatants, *p<0.05.