Figure 6.

The RNA secondary structure in the FKBP5 3′UTR influences miR-320a-binding efficiency. A, Schematics of SHAPE informed RNA secondary structures for the FKBP5 3′UTR in the presence of the major allele (top left) or the minor allele (bottom left) and schematics of predicted RNA secondary structures for the FKBP5 3′UTR with nucleotide changes that either increase base pairing in the vicinity of the miR-320a-binding site (base paired, top right) or decrease nucleotide complementarity (accessible, bottom right). The miR-320a-binding site is indicated by a red line on each schematic, and the seed region of miR-320a is indicated by an orange line. B, In vitro dual luciferase assays assessing miR-320a binding to the FKBP5 3′UTR in the presence of each construct represented by images in A. Error bars (SD) are derived from three independent experiments performed in duplicate.