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. 2018 Aug 8;46(17):8679–8688. doi: 10.1093/nar/gky704

Figure 5.

Figure 5.

Evaluation of triplex-forming ability of TFOs containing modified AP-ΨdC derivatives against multiple CG base pairs. (A) The hTERT promoter sequence (hTR) containing four CG inversion sites and sequences of the corresponding TFOs (HTR-T and HTR-Z). (B) The Cyclin D1 promoter sequence (Cy) containing three CG inversion sites and sequences of the corresponding TFOs (Cy-T and Cy-Z). (C) FAM-labeled hTR (32 bp; 100 nM) was incubated with increasing concentrations of each TFO (26 mer; 0–1000 nM) in the buffer containing 20 mM Tris–HCl, 2.5 mM MgCl2 and 2.5 mM spermidine at pH 7.5 and 37°C. Electrophoresis was performed with a 10% non-denaturing polyacrylamide gel at 4°C. Ks (106M−1) = [Triplex]/([TFO][Duplex]). (D) FAM-labeled Cy (30 bp; 100 nM) was incubated with increasing concentrations of each TFO (18 mer; 0–1000 nM) in the buffer containing 89 mM Tris–HCl, 10 mM MgCl2 at pH 7.4 and 37°C. Electrophoresis was performed with a 10% non-denaturing polyacrylamide gel at 4°C. Ks (106M−1) = [Triplex]/([TFO][Duplex]).