Table 2.
Primers used for transcript quantification by qPCR.
| Genea | Primer sequences (5'-3' )b |
|---|---|
| Hs/Rs miR-188-5p Stemloop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCTCC |
| Hs/Rs miR-188-5p F | GGTCCCATCCCTTGCATGGTG |
| Hs/Rs RNU6 Stemloop | GTCGTATCCAGTGCAGGGTCCGAGG |
| Hs/Rs RNU6 F | TGCGGGTGCTCGCTTCGGCAGC |
| Universal primer R | CCAGTGCAGGGTCCGAGGT |
| Hs E-cadherin F | CCACCAGATGACGATACCCG |
| Hs E-cadherin R | GCTTCAGAACCACTCCCCTC |
| Hs Vimentin F | GGGACCTCTACGAGGAGGAG |
| Hs Vimentin R | CGCATTGTCAACATCCTGTC |
| Hs α-SMA F | ACTGCCTTGGTGTGTGACAA |
| Hs α-SMA R | TCCCAGTTGGTGATGATGCC |
| Hs PTEN F | ACTCTTTATGCGCTGCGG |
| Hs PTEN R | ACAGCGGCTCAACTCTCAAA |
| Hs GAPDH F | CTCCTCCACCTTTGACGCTG |
| Hs GAPDH R | TCCTCTTGTGCTCTTGCTGG |
a Hs, human; Rs, rat.
b F, forward primer; R, reverse primer.