Fig 3.

Polydatin reduced NASH in MCD-fed mice through inhibition of oxidative stress. Mice were fed with MCD diet or control diet for 4 weeks. Mice fed with MCD diet were intraperitoneal injected with 5 mg/kg polydatin daily in the period of experiment (n=5/group). (A) The Representative fluorescent detection of DCFH-DA. (B) Representative immunofluorescent staining of NOX4. (C) Representative immunofluorescent staining of 4-HNE. (D-F) Quantification of positive staining areas was measured by Image J software. (G) The protein expression of NOX4 was examined by Western blot and analysis. ^#p < 0.05, ^####p < 0.0001 vs control mice; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs mice induced by MCD.