Table 1.
Primer sequences for real-time PCR
| Genes | Forward primer (5'-3') | Reverse primer (5'-3') |
|---|---|---|
| Mouse | ||
| IL-1β | TGCCACCTTTTGACAGTGATG | ATGTGCTGCTGCGAGATTTG |
| IL-6 | ACCAGAGGAAATTTTCAATAGGC | TGATGCACTTGCAGAAAACA |
| Col1α1 | TAGGCCATTGTGTATGCAGC | ACATGTTCAGCTTTGTGGACC |
| TGF-β1 | GTGGAAATCAACGGGATCAG | ACTTCCAACCCAGGTCCTTC |
| TIMP1 | AGGTGGTCTCGTTGATTTCT | GTAAGGCCTGTAGCTGTGCC |
| SREBP-1c | TAGAGCATATCCCCCAGGTG | GGTACGGGCCACAAGAAGTA |
| FAS | TCCTGGAACGAGAACACGATCT | GAGACGTGTCACTCCTGGACTTG |
| GAPDH | AGGAGTAAGAAACCCTGGAC | CTGGGATGGAATTGTGAG |
| Human | ||
| IL-1β | TTCGACACATGGGATAACGAGG | TTTTTGCTGTGAGTCCCGGAG |
| IL-6 | ACTCACCTCTTCAGAACGAATTG | CCATCTTTGGAAGGTTCAGGTTG |
| TNF-α | CCTGTAGCCCATGTTGTAGCAA | CCTTGAAGAGGACCTGGGAGTAG |
| SREBP-1c | GCCCCTGTAACGACCACTG | CAGCGAGTCTGCCTTGATG |
| FAS | AGATTGTGTGATGAAGGACATGG | TGTTGCTGGTGAGTGTGCATT |
| GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC |