Table 1.
The linker Primer sequence for PCR-DGGE and Real-time PCR
| Target bacteria | Primer Sequence (5¹-3¹) | |
|---|---|---|
| PCR-DGGE Primer | ||
| 341-F | CCTACGGGAGGCAGCAG | |
| 534-R | ATT ACCGCGGCTGCTGG | |
| 341FG | CGCCCGCCGCGCGCGGCGGCGCGGGGCGGGGGCACGGGGGGCCTACGGGAGG CAG CAG | |
| Real Time PCR Primer | ||
| Bifidobacterium (550 bp) | Bifid F | CTC CTGGAAACGGGTGG |
| Bifi-R | GGTGTTCTTCCCGATATCTACA | |
| Lactobacillus (250 bp) | Lact F | CTC AAA ACT AAACAAAGTTTC |
| Lact R | CTC AAA ACT AAACAAAGTTTC | |
| Bacteroides vulgatus (287bp) | BV- F | GCATCATGAGTCCGCATGTTC |
| BV-R | TCC ATA CCC GACTTT ATT CCTT | |
| Clostridium leptum (239bp) | C.lep-F | GCACAAGCAGTG GAG T |
| C.lep-R | CTTCCTCCGTTTTGTCAA | |