Figure 4.

Inhibition of cellular glucose metabolism blocks WST-8 reduction. (A~D) Increasing amount of inhibitors was used to treat HEK293 cells for 6 hours. Absorbance of WST-8 formazan was recorded after incubation for 1 hour. Data of GLUT inhibitor II (A), hexokinase inhibitor 2-DG (B), GAPDH inhibitor Iodoacetate (C) and G6PD inhibitor (DHEA) (D) were presented. (E) HEK293 cells were treated with inhibitors including GLUT inhibitor II (25μM), 2-DG (25mM), DHEA (62.5μM) for 6 hours. Phosphorylation of ACC on serine-79, Raptor on serine-792, S6K1 on threonine-389, Akt on threonine-308 and expression of protein interacting with NIMA (Pin1), beta-tubulin or GAPDH were determined by Western Blot.