Figure 5.

Inhibition of EGFR-PI3K-Akt pathway attenuates WST-8 reduction. (A~E) Increasing amount of inhibitors was used treat HEK293 cells for 6 hours. Absorbance of WST-8 formazan was recorded after incubation for 1 hour. Data of EGFR inhibitor (A), PI3K inhibitor LY294002 (B), Akt inhibitor VIII (C), mTORC1 inhibitor Rapamycin (D) and MEK inhibitor PD98059 were presented. Data of three independent replicates are presented as the mean+/-s.e.m., n=3. (F) HEK293 cells were treated with inhibitors including EGFR inhibitor (3 μM), LY294002 (20 μM), Akt inhibitor VIII (2 μM), Rapamycin (0.5 μM) and PD98059 (18 μM) for 6 hours. Phosphorylation of Akt on serine-473 or threonine-308, phosphorylation of S6K1 on threonine-389, expression of Pin1, tubulin and GAPDH were determined by Western Blot.