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. 2018 Aug 6;14(11):1445–1456. doi: 10.7150/ijbs.26335

Figure 7.

Figure 7

Downregulation of miR-485-3p in osteosarcoma cells was caused by the DNA hypermethylation of CpG islands. (A) The adjacent upstream of miR-485-3p had a CpG island. The genomic locus of the CpG island and miR-485-3p were shown. (B) AZA treatment significantly upregulated miR-485-3p levels. The hFOB1.19, U2OS and MG63 cells were treated with DMSO, 1 μM of AZA and 300 nM of TSA, respectively. The qRT-PCR was performed to measure miR-485-3p levels. **P < 0.001. (C) AZA treatment dramatically decreased DNA methylation of CpG island in U2OS and MG63 cells. The qMSP was performed to determine DNA methylation of CpG island in hFOB1.19, U2OS and MG63 cells. **P < 0.001. (D) AZA treatment dramatically decreased the CtBP1 level in U2OS and MG63 cells. The qRT-PCR was performed to determine CtBP1 levels in hFOB1.19, U2OS and MG63 cells. **P < 0.001. (E) AZA treatment significantly upregulated the CtBP1 target gene in U2OS and MG63 cells. The qRT-PCR was performed to determine CtBP1 targets, including Bax, Bim, PUMA, p16, p21, E-cadherin and PTEN in hFOB1.19, U2OS and MG63 cells. **P < 0.001.