Figure 5.

ComM exhibits 3-stranded branch migration activity on long DNA substrates in vitro. (A) Schematic for RecA-mediated strand exchange between linear double stranded φX174 (LDS) and circular single-stranded φX174 (CSS), which results in the formation of intermediates (INT) that can be resolved to nicked product (NP) if strand exchange commences to completion. Strand exchange reactions were deproteinated prior to complete strand exchange, and the resulting DNA was used to assess branch migration-dependent resolution of intermediate structures (INT). (B) Representative gel where deproteinated intermediates were incubated with the proteins indicated. (C) Three independent replicates of the assay described in B were quantified, and the relative abundance of the INT, NP, and LDS are shown as the mean ± SD.