Figure 6.

NMR analysis of the G4s. (A) fragments of 1H-NMR spectra (imino regions). (B) DOSY-NMR results. (C) Effects of salt and temperature on 3G_L1 imino signals. Conditions: 100 μM ON, 20 mM Tris–HCl (pH 7.6); rapid preannealing of 2G_L1 and 3G_L1. The control monomer TBA15 was preannealed rapidly to facilitate intramolecular folding, and the control tetramers (AG₄ and TG₆T) were preannealed slowly to facilitate intermolecular folding. KCl concentrations: 10 mM (A and B), 0 (left panel in C) or 20 mM (right panel in C).