Table 1.
G4 sequences, MS data, topologies and melting/annealing temperatures
| T m/Ta, ±1°C* | ||||||
|---|---|---|---|---|---|---|
| Code | Sequence | m/z [M+H]+, found (calculated) | G4 type | Hysteresis | 10 mM KCl, rapid preannealing | 200 mM KCl, slow preannealing |
| 2G_L0 | GGxGGxGGxGG | 3113 (3113) | Parallel | + | 67/ 45 | ≥85/NA |
| Alu-G4 | GGCGGAGGCGG | 3464 (3463) | Parallel | + | ∼55/NA | ≥85/55 |
| 2G_L1 | GGTGGTGGTGG | 3488 (3485) | Parallel | + | 49/NA | 62/55 |
| 2G_L3 | GGTTAGGTTAGGTTAGG | 5337 (5338) | Antiparallel | - | 36/NA | 48/48 |
| 3G_L0 | GGGxGGGxGGGxGGG | 4430 (4430) | Parallel | NA | ≥85 | ≥90 |
| 3G_L1 | GGGTGGGTGGGTGGG | 4800 (4802) | Parallel | NA | ≥85 | ≥90 |
| 3G_L3 | GGGTTAGGGTTAGGGTTAGGG | 6655 (6653) | Mixed, predominantly antiparallel | - | 56/NA | 74/74 |