Skip to main content
. 2018 Sep 25;141(10):2878–2894. doi: 10.1093/brain/awy237

Figure 2.

Figure 2

Label-free proteomics analysis identifies tRNA synthetases as UBA1-dependent proteins. (A) UBA1 expression in HEK293 cells used for proteomics screen showing UBA1 overexpression (OE) and knockdown (KD) compared to control. CoxIV and H3 = loading control. (B and C) Quantification of UBA1 overexpression (B) and knockdown (C). Ctrl = control; **P < 0.01, ***P < 0.001. (D) BioLayout clustering 3D representation of proteomic expression across UBA1 overexpression, control and UBA1 knockdown. Nodes represent proteins and edges represent similarity of expression between proteins and clusters of proteins with similar expression profiles are indicated by different colours. Cluster numbers are indicated. (E and F) Expression profile means ± SEM in log scale for the two UBA1-dependent clusters: cluster 1 (E) and cluster 4 (F). (G and H) IPA analysis showing the top network for UBA1 overexpression/control (G) and UBA1 knockdown/control (H), proteins highlighted in magenta are upregulated, proteins highlighted in green are downregulated. See also Supplementary Figs 2–5 and Supplementary Table 1.