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. 2018 Mar 29;46(12):6318–6329. doi: 10.1093/nar/gky233

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Edc3 alleviates autoinhibition and promotes RNA binding. (A) Plot of k_obs versus Dcp1:Dcp2_HLM-1 concentration in the absence (light blue) or presence (dark blue) of saturating concentrations of Edc3. Error bars represent population standard deviation, σ. (B) Plot of k_obs versus Dcp1:Dcp2_ext concentration in the absence (light gray) or presence (dark gray) of saturating concentrations of Edc3. Error bars represent population standard deviation, σ. (C) Comparison of fitted k_max (top) and K_m,app (bottom) values from panel A for Dcp1:Dcp2_HLM-1 with or without saturating Edc3 (colored as in panel A). There is not a significant change in k_max upon addition of Edc3 (as determined by unpaired t-test, see Supplementary Table S3) but K_m,app is 5-fold decreased. (D) Comparison of fitted k_max (top) and K_m,app (bottom) values from panel B for Dcp1:Dcp2_ext with or without saturating Edc3 (colored as in panel B). There is a 6-fold increase in k_max upon incubation with Edc3 and K_m,app decreases by 5-fold. Error bars are the population standard deviation, σ.