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Development of a novel algorithm IntMAP by integrating RNA-Seq and PAS-Seq dataset. (A) A schematic of the experimental workflow and algorithm to integrate RNA-Seq and PAS-Seq data. An algorithm integrating two datasets was developed for comprehensive profiling of 3′-UTR APA events. (B) A Venn diagram of 3′-UTR shortened transcripts in Tsc1−/− MEFs using IntMAP. The number of genes producing 3′-UTR shortened transcripts in each section of Venn diagram is indicated.
