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. 2018 Sep 4;7(9):299–308. doi: 10.1089/wound.2017.0775

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© Denise R. Cooper et al., 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Exosomes from hADSC CM promoted HDFa cell migration in ECIS wound healing assay. HDFa were grown to confluency on eight well ECIS plate and cells were killed using electrical voltage as described in the Materials and Methods section. Cells were then treated with control (unconditioned) media, bFGF (400 nM), 10 μg/mL exosome, and 20 μg/mL exosome. (A) Resistance values were measured every 120 s and% recovery was calculated as the difference in the resistance value of each time point and the resistance value of the first time point after wounding, divided by the resistance before wounding. Treatment with 10 μg/mL exosome increased recovery of TER mimicking the effect of cells treated with bFGF. Treatment with 20 μg/mL exosome further enhanced the recovery of TER compared to cells treated with bFGF. n = 3. (B) Cell migration rate was determined by formula v = r/t where r = 125 micrometer (radius of the microelectrode) and t = time to heal. n = 3. *p < 0.05 from control. Wounded HDFa cells treated with 20 μg/mL exosomes showed a significant twofold increase in cell migration compared to the bFGF treatment. bFGF, FGF2 or basic fibroblast growth factor; ECIS, electric cell-substrate impedance sensing; hADSC, human adipose-derived stem cell; TER, transcellular electrical resistance.