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. Author manuscript; available in PMC: 2018 Sep 27.
Published in final edited form as: Neurotox Res. 2015 Jul 23;28(4):302–318. doi: 10.1007/s12640-015-9548-6

Fig. 6.

Fig. 6

Western blot analysis on the expression of γ-glutamylcysteine synthetase (γ-GCLC) against 6-OHDA (a) and BSO with/withoutD-512 (b). For 6-OHDA experiment, PC12 cells were treated with 50 μM 6-OHDA for 6 and 24 h. For BSO experiment, PC12 cells were pretreated with 20 μM D512 for24 h followed by co-treatment with 1.56 μM BSO for another24 h. GAPDH was used as an internal control protein. Western blotting experiments are performed in triplicate or more and the quantifications are the average of these experiments