Fig. 7.

a Western blot analysis on the effect of D-512 in modulating MAP kinase (pERK). PC12 cells were pretreated with 10 μM D512 for 24 h followed by co-treatment with 75 μM 6-OHDA at different time points (0.5, 1, 2, and 4 h). GAPDH was used as an internal control protein. Western blotting experiments are performed in triplicate or more and the quantifications are the average of these experiments. One-way ANOVA analysis followed by Tukey’s multiple comparison post hoc test was performed (*p \ 0.01, ^#p \ 0.05 compared to control and ^#p \ 0.05 compared to 6-OHDA group for 2 h treatment; *p \ 0.01 compared to 6-OHDA group and ^#p \ 0.05 compared to D512 + 6-OHDA group for 4-h treatment). b Western blot analysis on the effect of D-512 in modulating MAP kinase (p-JNK). PC12 cells were pretreated with 10 μM D512 for 24 h followed by co-treatment with 75 μM 6-OHDA at different time points (0.5, 2, and 4 h). GAPDH was used as an internal control protein. Western blotting experiments are performed in triplicate or more and the quantifications are the average of these experiments