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. Author manuscript; available in PMC: 2019 Aug 17.
Published in final edited form as: ACS Chem Biol. 2018 Jun 15;13(8):2121–2129. doi: 10.1021/acschembio.8b00290

Figure 5. Kinetic analysis of recombinant BCO2 with apocarotenoid substrates.

Figure 5.

a. Michaelis Menten kinetic analyses of recombinant purified murine BCO2 incubated with increasing amounts (1.25–40 μM) of 3-hydroxy-APO8al (black) and APO8al (grey). Solid lines depict the Michaelis Menten curve with average parameters numerically calculated using MATLAB, and the dashed lines depict the 95% confidence interval. b. The table shows average +/-standard deviation values of Km, Kcat, Vmax, and Kcat/Km calculated from three independent kinetic analyses.