Table 2.
AZD3965 recovery, matrix effect, intra-day and inter-day accuracy and precision in mouse plasma. The measured concentrations represent the mean of triplicate measurements. The analyses were performed over 3 days.
| Intra-day | Inter-day | Recovery | Matrix Effect |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Standard Curve |
Nominal concentration (ng/mL) |
Mean (ng/mL) |
SD | Precision (CV%) |
Accuracy (%) |
Mean (ng/mL) |
SD | Precision (CV%) |
Accuracy (%) |
% | % | SD |
| Low | 10 | 10.3 | 0.06 | 0.56 | 103 | 10.1 | 0.225 | 2.23 | 101 | 101 | 103 | 4.18 |
| 150 | 153 | 4.93 | 3.22 | 102 | 148 | 4.36 | 2.94 | 98.9 | 101 | -- | -- | |
| 400 | 415 | 8.33 | 2.01 | 104 | 402 | 17.7 | 4.39 | 101 | 102 | 108 | 1.93 | |
| High | 1,000 | 1.04×103 | 15.3 | 1.47 | 104 | 1.01×103 | 30.1 | 2.98 | 101 | 103 | 101 | 1.84 |
| 6,000 | 6.00×103 | 124 | 2.07 | 100 | 5.96×103 | 144 | 2.42 | 99.4 | 101 | -- | -- | |
| 10,000 | 9.76×103 | 262 | 2.68 | 97.6 | 9.57×103 | 161 | 1.68 | 95.7 | 105 | 97.8 | 1.41 | |