Fig. 5.

Cytocompatibility assessment of decellularized ovarian scaffold. To assess soluble toxicity, HWJMSCs at a density of 1.0 × 10⁶, we seeded the cells onto each scaffold and cultured 1, 3 and 7 days. a MTT test showed that the cell proliferation on the decellularized scaffold was parallel with the control culture up to day 3, whereas, at longer culture intervals, a significant higher optical density value was detected in the seeded decellularized scaffolds than in the control group. Data are expressed as the mean ± standard error of the mean (SEM), *Indicates significant difference, p = 0.000). SEM micrographs revealed that the cells were attached on the surface of the scaffolds (b), and also they penetrated deep into the matrix (c). HWJMSCs human Wharton’s jelly mesenchymal stem cells