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. 2018 Sep 26;9:252. doi: 10.1186/s13287-018-0971-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Immunohistochemical assessments of POCs-DS (A-C) transplanted grafts. a Inhibin-α immunostaining for the detection of the granulosa cells. These cells (in brown) were scattered throughout the graft and some of them were organized into a follicle-like pattern (inset, a); Scale bars: 50 μm. b ER-positive brown cells were observed within the POCs-DS grafts. Strong staining intensity was observed in the cells located peripherally on the scaffolds (black arrows) compared with those located more centrally (inset, b); scale bars: 50 μm. c PR-positive cells were observed within the POCs-DS grafts as well; scale bar: 100 μm. (inset, c): PR-positive cells around an oocyte; scale bars: 50 μm. ER estrogen receptor, POCs-DS primary ovarian cells-seeded decellularized scaffolds, PR progesterone receptor