Fig. 1.

Metabolic gene expression in upper (USI) and lower small intestine (LSI) and the liver. All data are means ± SEM and were collected from mice after 12-week feeding either control (C), HFD (HF), or n-3 long-chain polyunsaturated fatty acid (LCPUFA)-enriched HFD (HF/n-3). a, b Gene expression of carnitine palmitoyl transferase 1a (Cpt1a), acyl-coenzyme A oxidase 1 (Acox1) and cytochrome P450, family 4, subfamily a, polypeptide 10 (Cyp4a10) representing proteins involved in mitochondrial and peroxisomal β-oxidation as well as ω-oxidation in USI (a; n = 7–11) and in LSI (b; n = 8–12). c Expression of genes operating in de novo lipogenesis, mitochondrial β-oxidation as well as ω-oxidation in liver (n = 10–12): Acetyl-CoA c arboxylase 2 (Acc2), enoyl-Coenzyme A hydratase (Lpbe) and Cyp4a10. RT-qPCR data were normalised to Hprt1, Gapdh and Actb (USI), Cypb and Hsp90αb1 (LSI) or Hprt1 and Actb (liver), and calculated relative to controls. *p < 0.05, **p < 0.01, ***p < 0.001, significant differences compared to control or between groups as indicated