Fig. 7.

Immunohistochemical analysis of macrophage-associated M1 and M2 marker expression in clusters of macrophages (MCL) of iBAT. Shown are microscopic images of paraffin sections of iBAT from mice fed control (a, d, g, j), HF (b, e, h, k) or HF/n-3 diet (c, f, i, l) after immunohistochemical staining followed by counterstaining with hematoxylin. Data for F4/80 (a, b, c), CD11c (d, e, f), CD206 (g, h, i) or CD301 (j, k, l) are presented. Positive-stained cells in MCL surrounding lipid droplets are highlighted by frames with continuous or dashed line, respectively. Inserts of the images (lower left corner) show one representative structure of each image at higher magnification. Scale bars indicate 50 μm for the section and 20 μm for the insert