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. 2018 Sep 26;9:249. doi: 10.1186/s13287-018-0997-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — In-vitro spermatogenesis using culture of seminiferous tubules (STs) or testicular cells from 3- or 6-day-old mice. a Schematic presentation of experimental procedures. b Stereomicroscopic appearance of colony formation and seminiferous tubules. Arrow indicates complete canalization of seminiferous tubules in the fourth week. Scale bars in STs = 1 mm and in colonies = 50 μm