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. 2018 Sep 26;9:249. doi: 10.1186/s13287-018-0997-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunofluorescence staining of frozen longitudinal sections of seminiferous tubules following 40 days of culture. Immunohistochemistry staining of frozen STs sections was performed by specific antibodies for different markers of germ cell development by immunofluorescence, including INTEGRIN α6, PLZF, ACROSIN (ACR), SCP3, and VIMENTIN. Scale bars =150 μm. PI propidium iodide