Table 4.
G protein-coupled receptors upregulated in the Y organ
| Annotation | Carma_YO_vs_Epi | Carma_YO | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cluster | LogFC | P | FDR | Cluster | LogFC | F | P | FDR | ||||
| A-B | D1 | D3 | D4 | |||||||||
| Gonadotropin-releasing hormone receptor | Cluster-3919.3796 | 11.07 | 2.14E-78 | 1.08E-74 | Cluster-136,339.18555 | −0.41 | −0.43 | 0.59 | −0.39 | 1.42 | 0.259 | 0.437 |
| Tachykinin-like peptides receptor 86C | Cluster-10,974.0 | 8.32 | 1.49E-15 | 4.06E-14 | Cluster-136,339.45038 | −1.12 | 0.26 | 0.24 | −0.94 | 0.38 | 0.824 | 0.913 |
| Relaxin receptor 1 | Cluster-3919.12767 | 6.20 | 5.35E-41 | 1.27E-38 | Cluster-131,500.3 | −0.19 | 0.25 | −0.29 | − 0.35 | 1.49 | 0.805 | 0.902 |
| Rhodopsin, G0-coupled | Cluster-3919.2920 | 4.30 | 8.58E-17 | 2.71E-15 | Cluster-62,481.4 | 0.27 | 0.37 | −1.21 | −2.04 | 8.31 | < 0.001 | 0.004 |
| Rhodopsin, G0-coupled | Cluster-3919.5747 | 2.60 | 2.83E-10 | 3.40E-09 | . | . | . | . | . | . | . | . |
| Probable G protein-coupled receptor Mth-like 1 | Cluster-69,371.0 | 2.31 | 4.77E-07 | 3.13E-06 | Cluster-136,339.38316 | 1.31 | −2.34 | 0.99 | 2.02 | 5.94 | 0.002 | 0.015 |
| Dopamine D2-like receptor | Cluster-3919.17236 | 2.18 | 0.002 | 6.76E-03 | Cluster-96,405.0 | 0.37 | −1.88 | −0.75 | −0.46 | 3.35 | 0.026 | 0.088 |
| Probable G protein-coupled receptor Mth-like 1 | Cluster-79,456.0 | 1.77 | < 0.001 | 1.58E-03 | Cluster-136,339.32542 | 3.81 | −0.95 | 1.61 | 3.14 | 3.73 | 0.017 | 0.066 |
| Opsin, ultraviolet-sensitive | Cluster-51,158.0 | 1.52 | < 0.001 | 1.53E-03 | Cluster-38,493.0 | 0.16 | 1.04 | 0.56 | 0.38 | 2.07 | 0.399 | 0.585 |
| 5-hydroxytryptamine receptor 4 | Cluster-27,180.0 | 1.26 | 0.014 | 3.23E-02 | Cluster-133,987.0 | −0.59 | −2.27 | −2.08 | −1.96 | 3.49 | 0.022 | 0.080 |
| G protein-coupled receptor 161 | Cluster-69,085.1 | 0.78 | 0.011 | 2.76E-02 | Cluster-31,544.1 | −0.40 | 0.89 | 1.54 | 1.16 | 2.59 | 0.062 | 0.164 |
Differential gene expression analysis statistics for clusters annotated with ‘GO:0004930 G protein-coupled activity’ and upregulated in YO relative to epidermis of C. maenas during inter-moult (C3–4), and across the moult cycle within the YO (Carma_YO-transcriptome) for those same GPCRs. Highlighted in bold are clusters differentially expressed over the moult cycle within the Y organ. ‘Rhodopsin, G0-coupled’ was clustered into two clusters within the Carma_YO_vs_Epi-transcriptome, but within a single cluster in the Carma_YO-transcriptome. For Carma_YO, logFC are relative to C3–4. The P-value should be read in conjunction with the false discovery rate (FDR)