Figure 1.

Effects of urea and KCl on binding of TLS/FUS and RGG3 to the G-quadruplex. (A) Schematic illustration of TLS/FUS and RGG3. Binding ability of DNA and RNA to TLS/FUS (B and D) and RGG3 (C and E) purified under different conditions was analyzed by EMSA. TLS/FUS and RGG3 purified in the presence of 150 mM KCl (lanes 2 and 6), 1 M urea and 150 mM KCl (lanes 3 and 7) or 1 M KCl (lanes 4 and 8) were incubated with DNA or RNA. EMSA was performed with TLS/FUS or RGG3 and either ³²P-labeled Htelo, ssDNA, TERRA or ssRNA. The nucleic acid–protein complexes were resolved by 6% polyacrylamide gel electrophoresis and visualized by autoradiography.