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. 2018 May 21;46(12):6057–6068. doi: 10.1093/nar/gky373

Figure 1.

Figure 1.

TgAP2X-5, an ApiAP2 protein is cell cycle-regulated and interacts with TgAP2XI-5. (A) Expression of the TgAP2X-5 protein (anti-HA antibody) during the tachyzoite cell cycle by confocal imaging. Anti-TgChromo1 antibody was used as marker of the cell cycle. DAPI was used to stain the nucleus. The cell cycle stage is indicated on the right side of the figure. Scale bar is indicated on the lower right side of each image. (B) TgAP2X-5 interacts with TgAP2XI-5. Nuclear extract of 3 × 108 parasites for the TgAP2X-5-myc alone (negative control) or the TgAP2X-5-HA/TgAP2X-5-myc strain or TgHP-HA/TgAP2X-5-myc strain were immunoprecipitated using anti-HA antibodies. The immunoblots were probed with anti-HA antibody (upper panel) to detect both HA-tagged proteins in the input and eluates. These immunoblots were reprobed with anti-myc antibody (lower panel) to detect the TgAP2XI-5-myc protein in the input and eluates.